GPA33 Antibody

• Catalog Number: orb1262466
• Category: Antibodies
• Description: GPA33 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This GPA33 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-293 amino acids from the C-terminal region of human GPA33.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 36 kDa
• Target: GPA33
• UniProt ID: Q99795
• NCBI: Q99795
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Cell surface A33 antigen, Glycoprotein A33, GPA33
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of GPA33 Antibody in K562, 293, MDA-MB231 cell line lysates (35 ug/lane)

GPA33 Antibody IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.