GOLPH2 Rabbit Polyclonal Antibody

SKU: orb4434

Description

GOLPH2 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=1ug/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GOLPH2 (21-120/401aa)
• Target: GOLM1
• Molecular Weight: 45 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Chromosome 9 open reading frame 155; Golgi membrane protein 1; Golgi membrane protein GP73; Golgi phosphoprotein 2; Golgi protein 73 kD; golgi protein 73kD; GOLM 1; GOLM1; GOLPH 2; GOLPH2; GP 73; GP73; PSEC0257; bA379P1.3; C9orf155.

(human/cell line) A431 lysates probed with GOLPH2 Polyclonal Antibody, Unconjugated (Catalog #orb4434) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37°C.

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-GOLPH2 antibody (orb4434), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human stomach tissue), Antigen retrieval by microwave in sodium citratebuffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RTfor 30 min, Antibody incubation with (GOLPH2) Polyclonal/MonoclonalAntibody, Unconjugated (orb4434) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue), Antigen retrieval by microwave in sodium citratebuffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RTfor 30 min, Antibody incubation with (GOLPH2) Polyclonal/MonoclonalAntibody, Unconjugated (orb4434) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: A549 (Human) Cell Lysate at 30 ug, MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-TBX1 (orb4434) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 45 kD.

Quick Database Links

Gene Symbol

GOLM1

UniProt

Q8NBJ4