GOLGA2 Antibody

• Catalog Number: orb1257543
• Category: Antibodies
• Description: GOLGA2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human GM130 (NP_004477.3).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 145kDa
• Target: GOLGA2
• UniProt ID: Q08379
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: GM13golgin subfamily A member 2, 130 kDa cis-Golgi
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Confocal immunofluorescence analysis of Hela cells using GM130 antibody (orb1257543) at dilution of 1:400. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using GM130 antibody (orb1257543) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunofluorescence analysis of HeLa cells using GM130 antibody (orb1257543) at dilution of 1:400 (60x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug GM130 antibody (orb1257543). Western blot was performed from the immunoprecipitate using GM130 antibody (orb1257543) at a dilution of 1:1000.