GM130 GOLGA2 Antibody (monoclonal, 4G3)

• Catalog Number: orb527062
• Category: Antibodies
• Description: GM130 GOLGA2 Antibody (monoclonal, 4G3)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4G3
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: E.coli-derived human GM130 recombinant protein (Position: E796-E913).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 5μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 140 kDa
• UniProt ID: Q08379
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Golgin subfamily A member 2; 130 kDa cis-Golgi mat
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-GM130 antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of GM130 using anti-GM130 antibody.Lane 1:human placenta tissue; 2:human K562 cell; 3:human CACO-2 cell; 4:human A549 cell; 5:human A431 cell; 6:human HEK293 cell.

IF analysis of GM130 using anti-GM130 antibody. GM130 was detected in immunocytochemical section of A431 cells.

IHC analysis of GM130 using anti-GM130 antibody. GM130 was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of GM130 using anti-GM130 antibody. GM130 was detected in paraffin-embedded section of human tonsil tissue.