Golden Syrian & Armenian Hamster IgG (H&L) Pre-Adsorbed Antibody

SKU: orb348522

Description

Golden Syrian & Armenian Hamster IgG (H&L) Pre-Adsorbed Antibody

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Golden Hamster, Hamster
• Application Notes:
  Anti-Golden Syrian & Armenian Hamster IgG antibody has been tested by ELISA and dot blot and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Armenian and Golden Syrian Hamster IgG, whole molecule
• Purity: Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian and Armenian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Golden Syrian Hamster IgG and Armenian Hamster IgG. No reaction was observed against Mouse or Rat serum proteins. Specificity was confirmed using ELISA at less than 1% cross reactivity.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.158 mg/mL
• Disclaimer: For research use only

Alternative Names

goat anti-Golden Syrian & Armenian Hamster IgG antibody, goat anti-Hamster IgG antibody

ELISA results of purified Goat Anti-Golden Syrian & Armenian Hamster IgG (Min X Mouse and Rat Serum Proteins) tested against purified Golden Syrian Hamster IgG and Armenian Hamster IgG. Each well was coated in duplicate with 1.0 µg of protein from different species. Golden Syrian Hamster IgG (Green Line), Armenian Hamster IgG (Red Line), Mouse IgG [p/n orb2652749] (Blue Line), and Rat IgG [orb2652744] (Purple Line). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Donkey Anti-Goat IgG HRP conjugated (p/n orb347032), and TMB substrate (p/n orb348651).