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| Catalog Number | orb20377 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal to XCL1 |
| Target | XCL1 / XCL2 |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Predicted Reactivity | Human |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | RDVVRSMDRKSNTRN |
| RRID | AB_10751666 |
| MW | 12.5; 12.6 |
| Tested applications | ELISA |
| Dilution range | ELISA: 1:16000, WB: 0.3 μg/ml |
| Application notes | XCL1 and CXL2 are almost identical and therefore this antibody is expected to recognize both proteins.ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 80kDa band in Human Peripheral Blood Mononucleocytes lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 12.5kDa according to NP_002986.1 and 12.6kDa according to 003166.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti XCL1 antibody, anti chemokine (C motif) ligan Read more... |
| Note | For research use only |
| Entrez | 6375 |
| Expiration Date | 12 months from date of receipt. |
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