UBE2L3 Antibody

• Catalog Number: orb18828
• Category: Antibodies
• Description: Goat polyclonal antibody to UBE2L3
• Target: UBE2L3
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EFTKKYGEKRPVD
• RRID: AB_10749778
• MW: 17.9
• Tested applications: ELISA, IF, WB
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 18kDa band observed in Hela and Jurkat lysates (predicted MW of 18kDa according to NP_003338). Recommended for use at 0.5-2 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti UBE2L3 antibody, anti ubiquitin-conjugating e
• Note: For research use only
• Entrez: 7332

EB05770 (0.01µg/ml) staining of HEK293 (A), HepG2 (B) and Jurkat (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

EB05770 (0.03µg/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunofluorescence staining of MCF7 (A) and Hela (B) cells with 5ug/ml EB05770 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.

EB05770 (0.01µg/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

EB05770 staining (0.5µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.