TRAF1 Antibody

SKU: orb19193

Description

Goat polyclonal antibody to TRAF1

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.3-1.0 μg/ml, IHC-P: 2.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 50-55kDa band observed in lysates of monocytic leukemia cell line U937 (calculated MW of 46.2kDa according to NP_005649.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pryhuber et al , Am J Respir Cell Mol Biol. 2000 Feb;22(2):150-6. PMID: 10657935). Recommended concentration: 0.3-1.0 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: TRAF1
• Molecular Weight: 46.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti TRAF1 antibody, anti TNF receptor-associated factor 1 antibody, anti EBI6 antibody, anti MGC:10353 antibody

Western blot analysis of U937 cell lysate using TRAF1 antibody

Immunohistochemical staining of Human Thymus using TRAF1 antibody

Immunohistochemical staining of Human Small Intestine using TRAF1 antibody

Quick Database Links

Gene Symbol

TRAF1

NCBI Gene ID

7185