TNNT1 Antibody

SKU: orb79336

Description

Goat polyclonal antibody to TNNT1

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.03-0.1 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Porcine
• Application Notes:
  WB: Preliminary experiments gave an approx 50kDa band in Human Skeletal Muscle lysates after 0.1μg/ml antibody staining.Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.9kDa according to NP_003274.3.The 50kDa band was successfully blocked by incubation with the immunizing peptide.We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates.Have any further splice variants/modified forms been reported.Experiment Notes: This antibody is expected to recognize all reported isoforms (NP_003274.3,NP_001119604.1,NP_001119605.1).

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Key Properties

• Clonality: Polyclonal
• Target: TNNT1
• Protein Sequence: EKAQELSDWIHQLE
• Molecular Weight: 32.9; 31.2; 30.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti TNNT1 antibody, anti troponin T type 1 (skeletal, slow) antibody, anti ANM antibody, anti FLJ98147 antibody, anti MGC104241 antibody, anti STNT antibody, anti TNT antibody, anti TNTS antibody, anti slow skeletal muscle troponin T antibody, anti troponin T, slow skeletal muscle antibody, anti troponin-T1, skeletal, slow antibody

Quick Database Links

Gene Symbol

TNNT1

NCBI Gene ID

7138