Goat anti-Tissue Factor Pathway Inhibitor Antibody

• Catalog Number: orb18320
• Category: Antibodies
• Description: Goat polyclonal antibody to TFPI
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Dilution range: ELISA: 1:64000, WB: 0.3-1 μg/ml
• Conjugation: Unconjugated
• MW: 35
• Target: Tissue Factor Pathway Inhibitor
• Entrez: 7035
• Protein Sequence: VKIAYEEIFVKNM
• RRID: AB_10746259
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti TFPI antibody, anti EPI antibody, anti LACI a
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 50kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 35.0kDa according to NP_006278.1). In transfected HEK293 transiently expressing TFPI bands of approx. 38kDa to 45kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18320 staining (0.3ug/ml) of Hela lysate (RIPA buffer, 35ug total protein cells per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

HEK293 overexpressing TFPI (RC219033) and probed with orb18320 (mock transfection in first lane), tested by Origene.