STK39/SPAK Antibody

SKU: orb20260

Description

Goat polyclonal antibody to STK39

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:8000, WB: 0.3-1 μg/ml, IHC-P: 3-5µg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  IHC: In paraffin embedded Human Cerebral Cortex shows textured staining in the neurons. Recommended concentration, 3-5μg/ml.WB: Approx 65kDa band observed in Human Brain (Cerebellum) lysates and in lysates of cell line Jurkat (calculated MW of 59.5kDa according to NP_037365.2). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: STK39 / SPAK
• Protein Sequence: SQEKSRRVKEENPE
• Molecular Weight: 59.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti STK39 antibody, anti serine threonine kinase 39 (STE20/SPS1 homolog, yeast) antibody, anti DCHT antibody, anti DKFZp686K05124 antibody, anti PASK antibody, anti SPAK antibody, anti Ste20-like protein kinase antibody, anti proline-alanine-rich STE20-related kinase antibody, anti small intestine SPAK-like kinase antibody

Western blot analysis of Jurkat lysate (35µg protein in RIPA buffer) using STK39 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded human cerebral cortex tissue using STK39 antibody

Western blot analysis of staining of Mouse (A) and Rat (B) Brain using STK39 antibody.

Quick Database Links

Gene Symbol

STK39 / SPAK

NCBI Gene ID

27347