SOX3 Antibody

SKU: orb20571

Description

Goat polyclonal antibody to SOX3

Research Area

Developmental Biology, Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:8000, WB: 0.5-1.5 μg/ml, IHC-P: 3-5µg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  IHC: In paraffin embedded Human Cerebrl Cortex shows nuclear staining at verious intensities along the neuronal cells. Recommended concentration, 3-5µg/ml.WB: Approx 42kDa band observed in Human Brain (Cerebellum, Cerebral Cortex and Frontal Cortex) lysates (calculated MW of 45.2kDa according to NP_005625.2). Recommended concentration: 0.5-1.5 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: SOX3
• Protein Sequence: DAASPLPGGRLHGVH
• Molecular Weight: 45.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti GHDX antibody, anti MRGH antibody, anti PHP antibody, anti PHPX antibody, anti SOXB antibody, anti SRY (sex determining region Y)-box 3 antibody, anti transcription factor SOX-3 antibody, anti SOX3 antibody

Western blot analysis of human cerebral cortex lysate (35µg protein in RIPA buffer) SOX3 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded human cerebral cortex tissue using SOX3 antibody

Quick Database Links

Gene Symbol

SOX3

NCBI Gene ID

6658