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Catalog Number | orb19518 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to SOD1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF, WB |
Reactivity | Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:8000, WB: 0.01-0.03 μg/ml, IHC-P: 10 μg/ml |
Conjugation | Unconjugated |
MW | 15.9 |
Target | SOD1 |
Entrez | 6647 |
Protein Sequence | SRKHGGPKDEERH |
RRID | AB_10767391 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti SOD1 antibody, anti superoxide dismutase 1, s Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 18kDa band observed in Mouse Brain and Rat Spinal Cord lysates (calculated MW of 15.9kDa according to Human NP_000445.1, Mouse NP_035564.1 and Rat NP_058746.1). Recommended concentration: 0.01-0.03 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb19518 (0.02ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of NIH3T3 (Lane 1), HEK293 (Lane 2), HepG2 (Lane 3) and MCF7 (Lane 4) lysates using SOD1 antibody
Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of using SOD1 antibody