SMAD4/MADH4 Antibody

SKU: orb18328

Description

Goat polyclonal antibody to SMAD4

Research Area

Cancer, Cardiovascular Disease, Stem Cells

Images & Validation

• Tested Applications: ELISA, IP
• Dilution Range: ELISA: 1:32000
• Predicted Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 60-65kDa band seen in mouse NIH-3T3 cell lysate. Recommended for use at 0.5-2 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: SMAD4 / MADH4
• Protein Sequence: HTMPIADPQPLD
• Molecular Weight: 60.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti MADH4 antibody, anti SMAD4 antibody, anti JIP antibody, anti DPC4 antibody, anti MAD, mothers against decapentaplegic homolog 4 (Drosophila) antibody, anti Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody, anti MAD (mothers against decapentaplegic, Drosophila) homolog 4 antibody, anti SMAD family member 4 antibody, anti OTTHUMP00000163548 antibody, anti SMAD, mothers against DPP homolog 4 antibody, anti deleted in pancreatic carcinoma locus 4 antibody, anti mothers against decapentaplegic homolog 4 antibody

orb18328 staining (0.5ug/ml) of NIH-3T3 cell lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Quick Database Links

Gene Symbol

SMAD4 / MADH4

NCBI Gene ID

4089