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SMAD4/MADH4 Antibody

SKU: orb18328

Description

Goat polyclonal antibody to SMAD4

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, IP
Dilution RangeELISA: 1:32000
Predicted ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 60-65kDa band seen in mouse NIH-3T3 cell lysate. Recommended for use at 0.5-2 μg/ml.

Key Properties

ClonalityPolyclonal
TargetSMAD4 / MADH4
Protein SequenceHTMPIADPQPLD
Molecular Weight60.4
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti MADH4 antibody, anti SMAD4 antibody, anti JIP antibody, anti DPC4 antibody, anti MAD, mothers against decapentaplegic homolog 4 (Drosophila) antibody, anti Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody, anti MAD (mothers against decapentaplegic, Drosophila) homolog 4 antibody, anti SMAD family member 4 antibody, anti OTTHUMP00000163548 antibody, anti SMAD, mothers against DPP homolog 4 antibody, anti deleted in pancreatic carcinoma locus 4 antibody, anti mothers against decapentaplegic homolog 4 antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
IP
Immunoprecipitation
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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