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Goat anti-SMAD4 / MADH4 Antibody

Catalog Number: orb18328

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb18328
CategoryAntibodies
DescriptionGoat polyclonal antibody to SMAD4
TargetSMAD4 / MADH4
ClonalityPolyclonal
Species/HostGoat
ConjugationUnconjugated
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Protein SequenceHTMPIADPQPLD
RRIDAB_10748843
MW60.4
Tested applicationsELISA, IP
Dilution rangeELISA: 1:32000
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 60-65kDa band seen in mouse NIH-3T3 cell lysate. Recommended for use at 0.5-2 μg/ml.
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti MADH4 antibody, anti SMAD4 antibody, anti JIP
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NoteFor research use only
Entrez4089
Goat anti-SMAD4 / MADH4 Antibody

orb18328 staining (0.5ug/ml) of NIH-3T3 cell lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.