Goat anti-SLC6A12 / BGT1 Antibody

• Catalog Number: orb79345
• Category: Antibodies
• Description: Goat polyclonal antibody to SLC6A12
• Target: SLC6A12 / BGT1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: CLDGSAGRNFGPSPT
• MW: 69.4
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 10 μg/ml
• Application notes: WB: Approx 70kDa band observed in Human Brain lysates from several regions (calculated MW of 69.4kDa according to NP_003035.3).Recommended concentration:1-3μg/ml. An additional band of unknown identity was also consistently observed at 27kDa.This band was successfully blocked by incubation with the immunizing peptide.We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates.Have any further splice variants/modified forms been reported.Experiment Notes: Reported variants represent identical protein:NP_001193860.1,NP_003035.3,NP_001116319.1,NP_001116320.1.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti BGT1 antibody, anti BGT-1 antibody, anti FLJ3
• Note: For research use only
• Entrez: 6539

Western blot analysis of human Olfactory bulb lysate using SLC6A12 antibody (35ug)