SLC12A2 Antibody

SKU: orb20466

Description

Goat polyclonal antibody to SLC12A2

Research Area

Cardiovascular Disease, Epigenetics

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC
• Dilution Range: ELISA: 1:64000, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave an approx 75kDa band in Human, Mouse and Rat Skeletal Muscle, Heart and Brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 131kDa according to NP_001037.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: SLC12A2
• Protein Sequence: NALQHSIRLSGVEDH
• Molecular Weight: 131, 130
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti basolateral Na-K-Cl symporter antibody, anti BSC antibody, anti BSC2 antibody, anti bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1 antibody, anti MGC104233 antibody, anti NKCC1 antibody, anti solute carrier family 12 (sodium/potassium/chloride transporters), member 2 antibody, anti SLC12A2 antibody

Immunohistochemical staining of Human Colon using SLC12A2 antibody

Quick Database Links

Gene Symbol

SLC12A2

NCBI Gene ID

6558