Goat anti-SLC12A2 Antibody

• Catalog Number: orb20466
• Category: Antibodies
• Description: Goat polyclonal antibody to SLC12A2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:64000, IHC-P: 3.75 μg/ml
• Conjugation: Unconjugated
• MW: 131, 130
• Target: SLC12A2
• Entrez: 6558
• Protein Sequence: NALQHSIRLSGVEDH
• RRID: AB_10753758
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti basolateral Na-K-Cl symporter antibody, anti
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave an approx 75kDa band in Human, Mouse and Rat Skeletal Muscle, Heart and Brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 131kDa according to NP_001037.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human Colon using SLC12A2 antibody