SH2D1A/SLAM associated protein Antibody

• Catalog Number: orb19242
• Category: Antibodies
• Description: Goat polyclonal antibody to SH2D1A
• Target: SH2D1A/SLAM associated protein
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Porcine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QYPVEKKSSARSTQ
• RRID: AB_10753333
• MW: 14.2; 13.9
• Tested applications: ELISA, IP, WB
• Dilution range: ELISA: 1:32000, WB: 0.2-0.6 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 16kDa band observed in Human Lymph Node lysates (calculated MW of 14.2kDa according to NP_002342.1). In transfected HEK293 transiently expressing SH2D1A a band of approx. 17kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti SH2D1A antibody, anti SH2 domain protein 1A,
• Note: For research use only
• Entrez: 4068

orb19242 (0.2ug/ml) staining of human lymph node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19242 overexpressing SH2D1A (RC204723) and probed with orb19242 (mock transfection in first lane), tested by Origene.