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Catalog Number | orb19051 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to SART3 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Dilution range | ELISA: 1:16000, WB: 0.01-0.03 μg/ml |
Conjugation | Unconjugated |
MW | 110 |
Target | SART3 |
Entrez | 9733 |
Protein Sequence | MSNADFAKLFLRK |
RRID | AB_10753009 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti SART3 antibody, anti squamous cell carcinoma Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 170kDa band observed in lysates of cell lines Daudi, Jurkat and MOLT4 (calculated MW of 111kDa according to NP_055521.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yang et al, Cancer Res. 1999 Aug 15;59(16):4056-63; PMID: 10463607). Recommended concentration: 0.01-0.03 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb19051 staining (0.01ug/ml) of Jurkat cell lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.