ROBO3 (internal) Antibody

• Catalog Number: orb125008
• Category: Antibodies
• Description: Goat polyclonal antibody to ROBO3
• Target: ROBO3 (internal)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: RKDGARLKEEEGR
• MW: 148
• Tested applications: ELISA, FC
• Dilution range: ELISA: 1:64000, IHC-P: 10ug/ml
• Application notes: Notes: Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml. This antibody was successfully used on rheumatoid arthritis synovial fibroblasts (RASF): Denk et al (2010) PMID: 20298552.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ROBO3 antibody, anti roundabout, axon guidanc
• Research Area: Neuroscience
• Note: For research use only
• Entrez: 64221
• Expiration Date: 12 months from date of receipt.

References

Denk, Alexandra E. et al. Slit3 inhibits Robo3-induced invasion of synovial fibroblasts in rheumatoid arthritis Arthritis Res Ther, 12, R45 (2010)

PubMed: 20298552