Robo1/DUTT1 (mouse) Antibody

SKU: orb19340

Description

Goat polyclonal antibody to Robo1

Research Area

Developmental Biology, Epigenetics, Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.05-0.2 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Mouse
• Predicted Reactivity: Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave bands at approx 80kDa and 40kDa in lysates of Mouse Brain and Rat Brain after 0.03 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 176kDa according to NP_062286.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Robo1 / DUTT1 (mouse)
• Protein Sequence: QKARPAKKQKHQ
• Molecular Weight: 176
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Robo1 antibody, anti roundabout homolog 1 (Drosophila) antibody, anti AW494633 antibody, anti AW742721 antibody, anti DUTT1 antibody, anti roundabout homolog 1 antibody

Western blot analysis of Mouse Brain lysate using Robo1 antibody

Immunohistochemical staining of Mouse Testis using Robo1 antibody

Quick Database Links

Gene Symbol

Robo1 / DUTT1 (mouse)