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Goat anti-Robo1 / DUTT1 (mouse) Antibody

Catalog Number: orb19340

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb19340
CategoryAntibodies
DescriptionGoat polyclonal antibody to Robo1
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, IHC, WB
ReactivityCanine, Mouse, Rat
Dilution rangeELISA: 1:128000, WB: 0.05-0.2 μg/ml, IHC-P: 5 μg/ml
ConjugationUnconjugated
MW176
TargetRobo1 / DUTT1 (mouse)
Protein SequenceQKARPAKKQKHQ
RRIDAB_10921166
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti Robo1 antibody, anti roundabout homolog 1 (Dr
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NoteFor research use only
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave bands at approx 80kDa and 40kDa in lysates of Mouse Brain and Rat Brain after 0.03 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 176kDa according to NP_062286.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Expiration Date12 months from date of receipt.
Goat anti-Robo1 / DUTT1 (mouse) Antibody

Western blot analysis of Mouse Brain lysate using Robo1 antibody

Goat anti-Robo1 / DUTT1 (mouse) Antibody

Immunohistochemical staining of Mouse Testis using Robo1 antibody