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Catalog Number | orb19276 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to ROBO1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC |
Reactivity | Bovine, Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:8000, WB: 1 μg/ml, IHC-P: 2 μg/ml |
Conjugation | Unconjugated |
MW | 181; 177 |
Target | ROBO1 / DUTT1 (Internal) |
Entrez | 6091 |
Protein Sequence | GDVDLSNKINEMK |
RRID | AB_10755911 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti ROBO1 antibody, anti DUTT1 antibody, anti rou Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 250kDa band observed in Human Endothelial Cell line HUVEC lysates (calculated MW of 181kDa according to NP_002932.1). Some of the observed proteolytic breakdown products are also found in Human, Mouse and Rat Brain lysates. Recommended concentration: 1-3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of HUVEC lysate using ROBO1 antibody
Immunohistochemical staining of Human Liver using ROBO1 antibody