Goat anti-RNF35 / TRIM40 Antibody

• Catalog Number: orb18488
• Category: Antibodies
• Description: Goat polyclonal to RNF35
• Target: RNF35 / TRIM40
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: GVSELLLQPPQKL
• RRID: AB_10748080
• MW: 26.1
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 0.5-2 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 45kDa band observed in Human Brain lysates at 0.5-1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that the band we observe is different from the predicted size of approx. 26kDa according to NP_619645. Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti TRIM40 antibody, anti tripartite motif-contai
• Note: For research use only
• Entrez: 135644

Western blot analysis of Human Placenta lysate using RNF35 antibody