RICTOR Antibody

• Catalog Number: orb19014
• Category: Antibodies
• Description: Goat polyclonal antibody to RICTOR
• Target: RICTOR
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KQPIVDTSAES
• RRID: AB_10747523
• MW: 192
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:4000, WB: 0.1 μg/ml, IHC-P: 2-3µg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.IHC: In paraffin embedded Kidney shows strong cytoplasmic staining of epithelial cells in distal convoluted tubules. Recommended concentration, 2-3ug/ml.WB: Preliminary experiments gave an approx 38kDa band in Human Lung Carcinoma A549 cell line and cervix epitheloid carcinoma cell line HeLa lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 192kDa according to NP_689969.2. The 38kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti RICTOR antibody, anti Rictor antibody, anti K
• Research Area: Developmental Biology, Epigenetics, Infectious Dis
• Note: For research use only
• Entrez: 253260

orb19014 (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.