RICTOR Antibody

SKU: orb19014

Description

Goat polyclonal antibody to RICTOR

Research Area

Developmental Biology, Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:4000, WB: 0.1 μg/ml, IHC-P: 2-3µg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.IHC: In paraffin embedded Kidney shows strong cytoplasmic staining of epithelial cells in distal convoluted tubules. Recommended concentration, 2-3ug/ml.WB: Preliminary experiments gave an approx 38kDa band in Human Lung Carcinoma A549 cell line and cervix epitheloid carcinoma cell line HeLa lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 192kDa according to NP_689969.2. The 38kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: RICTOR
• Protein Sequence: KQPIVDTSAES
• Molecular Weight: 192
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti RICTOR antibody, anti Rictor antibody, anti KIAA1999 antibody, anti AVO3 antibody, anti MGC39830 antibody, anti likely ortholog of mouse TORC2-specific protein AVO3 (S. cerevisiae) antibody, anti mAVO3 antibody, anti pianissimo antibody, anti DKFZp686B11164 antibody, complex 2 antibody, anti PIA antibody, anti mAVO3 antibody, anti TORC2-specific protein AVO3 antibody

orb19014 (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

RICTOR

NCBI Gene ID

253260