Radixin Antibody

SKU: orb19264

Description

Goat polyclonal antibody to RDX

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:4000, WB: 0.01-0.03 μg/ml, IHC-P: 2.5 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 80kDa band observed in Human Lymph Node, Tonsil and Spleen lysates (calculated MW of 68.6kDa according to NP_002897.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Magendantz et al, J Biol Chem. 1995 Oct 27;270(43):25324-7.; PMID: 7592691). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Radixin
• Protein Sequence: QARDETKKTQND
• Molecular Weight: 68.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti RDX antibody, anti radixin antibody

Western blot analysis of Human Tonsil lysate using RDX antibody

Western blot analysis of NIH3T3 (Lane 1), HeLa (Lane 2), Jurkat (Lane 3), and A431 (Lane 4) lysates using RDX antibody

Immunohistochemical staining of Human Kidney using RDX antibody

Immunohistochemical staining of Human Placenta using RDX antibody

Quick Database Links

Gene Symbol

Radixin

NCBI Gene ID

5962