Goat Anti-Rabbit IgG H&L, Cy3 conjugated

SKU: orb868589

Description

Goat Anti-Rabbit IgG H&L, Cy3 conjugated

Images & Validation

• Tested Applications: FC, ICC, IF
• Dilution range: IF=1:200-1000, Flow-Cyt=1:50-200, ICC/IF=1:100-1000
• Reactivity: Rabbit

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Native rabbit IgG
• Purification: Affinity purified by Protein G, nonspecific adsorbed

Storage & Handling

• Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 10 mM TBS (pH=7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% glycerol.
• Concentration: 2.0 mg/ml
• Disclaimer: For research use only

Alternative Names

Goat Anti-Rabbit IgG H&L (Cy3); Immunoglobulin G;

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (c-Maf) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (c-Maf) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (c-Maf) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.