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Goat anti-PXR / NR1I2 Antibody

Catalog Number: orb20145

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb20145
CategoryAntibodies
DescriptionGoat polyclonal antibody to PXR
TargetPXR / NR1I2
ClonalityPolyclonal
Species/HostGoat
ConjugationUnconjugated
ReactivityHuman
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Protein SequenceEQFAITLKSYIECNR
RRIDAB_10752066
MW49.8; 53.9; 45.8
Tested applicationsELISA, IHC, WB
Dilution rangeELISA: 1:4000, WB: 1-3 μg/ml, IHC-P: 3.75 μg/ml
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx. 48kDa band observed in lysates of cell line HepG2 (calculated MW of 49.8kDa according to NP_003880.3). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesAnti-Nr1i2 antibody, anti-NR1I2_HUMAN antibody, an
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NoteFor research use only
Entrez8856
Goat anti-PXR / NR1I2 Antibody

orb20145 (1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.