Goat anti-PXR / NR1I2 Antibody

• Catalog Number: orb20145
• Category: Antibodies
• Description: Goat polyclonal antibody to PXR
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:4000, WB: 1-3 μg/ml, IHC-P: 3.75 μg/ml
• Conjugation: Unconjugated
• MW: 49.8; 53.9; 45.8
• Target: PXR / NR1I2
• Entrez: 8856
• Protein Sequence: EQFAITLKSYIECNR
• RRID: AB_10752066
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Anti-Nr1i2 antibody, anti-NR1I2_HUMAN antibody, an
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx. 48kDa band observed in lysates of cell line HepG2 (calculated MW of 49.8kDa according to NP_003880.3). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb20145 (1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.