Goat anti-PU.1 Antibody

• Catalog Number: orb19964
• Category: Antibodies
• Description: Goat polyclonal antibody to SPI1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Rat
• Dilution range: ELISA: 1:32000, WB: 0.03-0.1 μg/ml, IHC-P: 3.75 μg/ml
• Conjugation: Unconjugated
• MW: 31.2; 31.1
• Target: PU.1
• Entrez: 6688
• Protein Sequence: DLYQRQTHEYY
• RRID: AB_10751740
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti spleen focus forming virus (SFFV) proviral in
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 40kDa band observed in lysates of DAUDI and K562 (calculated MW of 31.2kDa according to NP_001074016.1 and of 31.1kDa according to NP_003111.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Harendza S et al, J Biol Chem. 2000 Jun 30;275(26):19552-9.; PMID: 10867017). Recommended concentration: 0.03-0.1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19964 (0.03ug/ml) staining of DAUDI lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Colon using SPI1 antibody

Immunohistochemical staining of Human Spleen using SPI1 antibody