PU.1 Antibody

SKU: orb19964

Description

Goat polyclonal antibody to SPI1

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.03-0.1 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 40kDa band observed in lysates of DAUDI and K562 (calculated MW of 31.2kDa according to NP_001074016.1 and of 31.1kDa according to NP_003111.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Harendza S et al, J Biol Chem. 2000 Jun 30;275(26):19552-9.; PMID: 10867017). Recommended concentration: 0.03-0.1 μg/ml.

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Key Properties

• Clonality: Polyclonal
• Target: PU.1
• Protein Sequence: DLYQRQTHEYY
• Molecular Weight: 31.2; 31.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti spleen focus forming virus (SFFV) proviral integration oncogene spi1 antibody, anti hCG_25181 antibody, anti SFPI1 antibody, anti SPI-1 antibody, anti SPI-A antibody, anti 31 kDa transforming protein antibody

Quick Database Links

Gene Symbol

PU.1

NCBI Gene ID

6688