PSME2 Antibody

• Catalog Number: orb19476
• Category: Antibodies
• Description: Goat polyclonal antibody to PSME2
• Target: PSME2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: NLEKIVNPKGEEKP
• RRID: AB_10748405
• MW: 27.4
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.5-1 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 27kDa band observed in Human Spleen lysates (calculated MW of 27.4kDa according to NP_002809.2). Recommended concentration: 0.5-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PSME2 antibody, anti proteasome (prosome, mac
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 5721

orb19476 (0.5ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19476 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.