Prolyl Endopeptidase/PREP Antibody

• Catalog Number: orb19233
• Category: Antibodies
• Description: Goat polyclonal antibody to PREP
• Target: Prolyl Endopeptidase / PREP
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QYIVGRSRKQSNP
• RRID: AB_10762240
• MW: 80.7
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:16000, WB: 0.3 μg/ml, IHC-P: 3.75 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 45kDa band in Human Brain, Lymph Node, Spleen and Testis lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 80.7kDa according to NP_002717.3. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PREP antibody, anti prolyl endopeptidase anti
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 5550

Immunohistochemical staining of Human Kidney using PREP antibody