Prolyl Endopeptidase/PREP Antibody

SKU: orb19233

Description

Goat polyclonal antibody to PREP

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:16000, WB: 0.3 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 45kDa band in Human Brain, Lymph Node, Spleen and Testis lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 80.7kDa according to NP_002717.3. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

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Key Properties

• Clonality: Polyclonal
• Target: Prolyl Endopeptidase / PREP
• Protein Sequence: QYIVGRSRKQSNP
• Molecular Weight: 80.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PREP antibody, anti prolyl endopeptidase antibody, anti HGNC:9358 antibody, anti MGC16060 antibody, anti PE antibody, anti PEP antibody, anti OTTHUMP00000040498 antibody, anti dJ355L5.1 (prolyl endopeptidase) antibody, anti post-proline cleaving enzyme antibody

Quick Database Links

Gene Symbol

Prolyl Endopeptidase / PREP

NCBI Gene ID

5550