PRKCB Antibody

• Catalog Number: orb107663
• Category: Antibodies
• Description: Goat polyclonal to PRKCB
• Target: PRKCB
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DKEFTRQPVELT
• MW: 76.9; 77.0
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 1-2µg/ml
• Application notes: WB: Approx 80kDa band observed in lysates of Human Placenta and Rat Brain (calculated MW of 76.9kDa according to NP_997700.1). Recommended concentration: 0.1-0.3µg/ml.. IHC: In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration, 1-2µg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PRKCB antibody
• Note: For research use only
• Entrez: 5579
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Human Placenta lysate (35ug protein in RIPA buffer) using PRKCB antibody

Western blot analysis of Human Placenta (Lane 1) and Rat Brain (Lane 2) lysates using PRKCB antibody

Immunohistochemical staining of Human Spleen using PRKCB antibody