POU3F3/BRN1/OCT8 Antibody

SKU: orb20546

Description

Goat polyclonal antibody to POU3F3

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:4000, WB: 1-3 μg/ml, IF/ICC: 10μg/ml, FACS: 10ug/ml
• Reactivity: Mouse
• Predicted Reactivity: Human, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 48kDa band observed in Mouse and Rat Spinal Cord lysates (calculated MW of 50.3kDa according to Human NP_006227.1 and 50.2kDa according to Mouse NP_032926.2). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: POU3F3 / BRN1 / OCT8
• Protein Sequence: HMLSHAHQWVTAL
• Molecular Weight: 50.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Brain-1 antibody, anti BRN1 antibody, anti OTF8 antibody, anti POU class 3 homeobox 3 antibody, anti POU domain, class 3, transcription factor 3 antibody, anti POU3F3 antibody, anti OTF-8 antibody, anti brain-specific homeobox/POU domain protein 1 antibody, anti brn-1 antibody, anti oct-8 antibody, anti octamer-binding protein 8 antibody, anti octamer-binding transcription factor 8 antibody

Flow cytometric analysis of paraformaldehyde fixed Neuro-2a cells using POU3F3 antibody.

Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells using POU3F3 antibody.

Western blot analysis of Mouse Spinal Cord (A) and Brain (B) lysate using POU3F3 antibody.

Quick Database Links

Gene Symbol

POU3F3 / BRN1 / OCT8

NCBI Gene ID

5455