Goat anti-PMSCL1 Antibody

SKU: orb20026

Description

Goat polyclonal antibody to EXOSC9

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:64000, WB: 0.3-1 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 75kDa band observed in lysates of cell line Jurkat (calculated MW of 50.8kDa according to NP_001029366.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Alderuccio, J Exp Med. 1991 Apr 1;173(4):941-52; PMID: 2007859). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PMSCL1
• Protein Sequence: RTQTTSAKQEKAP
• Molecular Weight: 50.8; 48.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti EXOSC9 antibody, anti exosome component 9 antibody, anti PM/Scl-75 antibody, anti PMSCL1 antibody, anti RRP45 antibody, anti Rrp45p antibody, anti p5 antibody, anti p6 antibody, anti P75 polymyositis-scleroderma overlap syndrome associated autoantigen antibody, anti PMSCL autoantigen, 75kD antibody, anti autoantigen PM/Scl 1 antibody, anti polymyositis/scleroderma autoantigen 1 (75kD) antibody, anti polymyositis/scleroderma autoantigen 1, 75kDa antibody

orb20026 (0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

PMSCL1

NCBI Gene ID

5393