Goat anti-Phorbolin 1 / APOBEC3A Antibody

SKU: orb19763

Description

Goat polyclonal antibody to APOBEC3A

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 0.1-1ug/ml, IHC-P: 3-6 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave an approx 28kDa band in Human Spleen, Tonsil and Peripheral Blood Mononucleocyte lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 23.0kDa according to NP_663745.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Phorbolin 1 / APOBEC3A
• Molecular Weight: 23
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti APOBEC3A antibody, anti apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A antibody, anti ARP3 antibody, anti PHRBN antibody, anti bK150C2.1 antibody, anti phorbolin 1 antibody

Immunohistochemical staining of Human Tonsil using APOBEC3A antibody

Western blot analysis of Human Spleen (A), Tonsil (B) and PBM (C) lysate using APOBEC3A antibody

Flow cytometric analysis of HEK293 cells using APOBEC3A antibody

Immunofluorescence analysis of A549 Cells using APOBEC3A antibody

Immunofluorescence analysis of A431 cells using APOBEC3A antibody

Quick Database Links

Gene Symbol

Phorbolin 1 / APOBEC3A

NCBI Gene ID

200315