Goat anti-Phorbolin 1 / APOBEC3A Antibody

• Catalog Number: orb19763
• Category: Antibodies
• Description: Goat polyclonal antibody to APOBEC3A
• Target: Phorbolin 1 / APOBEC3A
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: TSNFNNGIGRHKTY
• RRID: AB_10747865
• MW: 23
• Tested applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 0.1-1ug/ml, IHC-P: 3-6 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave an approx 28kDa band in Human Spleen, Tonsil and Peripheral Blood Mononucleocyte lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 23.0kDa according to NP_663745.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti APOBEC3A antibody, anti apolipoprotein B mRNA
• Note: For research use only
• Entrez: 200315

Immunohistochemical staining of Human Tonsil using APOBEC3A antibody

Western blot analysis of Human Spleen (A), Tonsil (B) and PBM (C) lysate using APOBEC3A antibody

Flow cytometric analysis of HEK293 cells using APOBEC3A antibody

Immunofluorescence analysis of A549 Cells using APOBEC3A antibody

Immunofluorescence analysis of A431 cells using APOBEC3A antibody