PDPN Antibody

SKU: orb20288

Description

Goat polyclonal to PDPN

Research Area

Cardiovascular Disease, Developmental Biology, Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:1000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine
• Application Notes:
  This antibody is expected to recognize all isoforms (NP_006465.3; NP_938203.2; NP_001006625.1; NP_001006626.1).ELISA: Peptide ELISA: antibody detection limit dilution 1:1000.WB: Experiments gave bands at approx 25kDa and 37kDa in Human Brain (Cerebellum) lysates after 1 μg/ml antibody staining. The 37kDa band corresponds to earlier findings in literature with different antibodies (Boucherot et al, Nephrol Dial Transplant. 2002 Jun;17(6):978-84; PMID: 12032185). This protein has a calculated MW of 24.9kDa according to NP_006465.3. Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PDPN
• Protein Sequence: EKVDGDTQTTVEKD
• Molecular Weight: 24.9; 24.7; 12.4; 12.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PDPN antibody, anti podoplanin antibody, anti AGGRUS antibody, anti GP36 antibody, anti GP40 antibody, anti Gp38 antibody, anti HT1A-1 antibody, anti OTS8 antibody, anti PA2.26 antibody, anti T1A antibody, anti T1A-2 antibody, anti OTTHUMP00000009640 antibody, anti OTTHUMP00000044504 antibody, anti glycoprotein, 36-KD antibody, anti hT1alpha-1 antibody, anti hT1alpha-2 antibody, anti lung type I cell membrane associated glycoprotein antibody, anti lung type-I cell membrane-associated glycoprotein antibody, anti lung type-I cell membrane-associated glycoprotein (T1A-2) antibody

orb20288 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

PDPN

NCBI Gene ID

10630