PCK2/PEPCK-M Antibody

SKU: orb19100

Description

Goat polyclonal antibody to PCK2

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:4000, WB: 2-4 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:1000.WB: Approx 70kDa band observed in Human Liver lysates and in lysates of cell line HepG2 (calculated MW of 70.7kDa according to NP_004554.2). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PCK2 / PEPCK-M
• Protein Sequence: KPWKPGDKEPCAH
• Molecular Weight: 70.7; 56.0
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PCK2 antibody, anti PEPCK-M antibody, anti phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody, anti HGNC:8725 antibody, anti PEPCK antibody, anti PEPCK2 antibody, anti PEP carboxykinase antibody, anti mitochondrial phosphoenolpyruvate carboxykinase 2 antibody, anti phosphoenolpyruvate carboxylase antibody, anti phosphopyruvate carboxylase antibody

Western blot analysis of HepG2 lysate using PCK2 antibody

Western blot analysis of Human Liver lysate using PCK2 antibody

Quick Database Links

Gene Symbol

PCK2 / PEPCK-M

NCBI Gene ID

5106