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Catalog Number | orb18313 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to ORC3L |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine, Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 3.75 μg/ml |
Conjugation | Unconjugated |
MW | 82.3; 82.3; 65.9 |
Target | ORC3L |
Entrez | 23595 |
Protein Sequence | QKTDHVARLTWGGC |
RRID | AB_10750068 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti ORC3L antibody, anti origin recognition compl Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 75kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 82.3kDa according to NP_862820.1 and NP_036513.2). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Hela lysate using ORC3L antibody.
Immunohistochemical staining of Human Cortex using ORC3L antibody