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NIPBL (aa889-902) Antibody

SKU: orb20548

Description

Goat polyclonal antibody to NIPBL

Research Area

Stem Cell & Developmental Biology

Images & Validation

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Tested ApplicationsELISA, IF, IHC
Dilution RangeELISA: 1:32000, IHC-P: 2 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 316kDa according to NP_597677.2 or 304kDa according to NP_056199.2. Therefore we cannot recommend an optimal concentration and the antibody is an aspiring product. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

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ClonalityPolyclonal
TargetNIPBL (aa889-902)
Protein SequenceRPDSPRVKQGDSNK
Molecular Weight316; 304
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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anti CDLS antibody, anti CDLS1 antibody, anti DKFZp434L1319 antibody, anti FLJ11203 antibody, anti FLJ12597 antibody, anti FLJ13354 antibody, anti FLJ13648 antibody, anti FLJ44854 antibody, anti IDN3 antibody, anti IDN3-B antibody, anti Nipped-B homolog (Drosophila) antibody, anti nipped-B-like antibody, anti Scc2 antibody, anti SCC2 homolog antibody, anti sister chromatid cohesion 2 homolog antibody, anti NIPBL antibody
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

NCBI Gene Details

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No NCBI Gene data available

Protocol Information

IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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