NANOG Antibody

SKU: orb19063

Description

Goat polyclonal antibody to NANOG

Research Area

Developmental Biology, Stem Cells

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:64000, WB: 0.03-0.1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.1). Recommended concentration: 0.03-0.1 μg/ml.Experiment Notes: Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green). Immunofluorescence: see also publication Raya A. et al., Nature. 2009 Jul 2;460(7251):53-9. Epub 2009 May 31. (PMID: 19483674). This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: NANOG
• Protein Sequence: QNQRMKSKRWQKNN
• Molecular Weight: 34.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti NANOG antibody, anti Nanog homeobox antibody, anti HGNC:20857 antibody, anti homeobox transcription factor Nanog antibody, anti homeobox transcription factor Nanog-delta 48 antibody

Western blot analysis of Human Ovary lysate using NANOG antibody

Immunofluorescence analysis of Human Keratinocytes using NANOG antibody

Quick Database Links

Gene Symbol

NANOG

NCBI Gene ID

79923