MURF1/TRIM63 (internal) Antibody

SKU: orb19113

Description

Goat polyclonal antibody to TRIM63

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution range: ELISA: 1:32000, WB: 0.3 μg/ml, IHC-P: 1.25 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 75kDa band in human heart and skeletal muscle lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.2kDa according to NP_115977.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: MURF1 / TRIM63 (internal)
• Molecular Weight: 40.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti TRIM63 antibody, anti tripartite motif-containing 63 antibody, anti HGNC:16007 antibody, anti FLJ32380 antibody, anti MURF1 antibody, anti MURF2 antibody, anti SMRZ antibody

Immunohistochemical staining of Human Skeletal Muscle using TRIM63 antibody

Quick Database Links

Gene Symbol

MURF1 / TRIM63 (internal)

NCBI Gene ID

84676