Goat anti-MTHFD1 Antibody

• Catalog Number: orb20061
• Category: Antibodies
• Description: Goat polyclonal antibody to MTHFD1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: ELISA: 1:1000, WB: 1-3 μg/ml
• Conjugation: Unconjugated
• MW: 102
• Target: MTHFD1
• Entrez: 4522
• Protein Sequence: RGDLNDCFIPCTPK
• RRID: AB_10748566
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti MTHFD1 antibody, anti methylenetetrahydrofola
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:1000.WB: Approx. 110kDa band observed in Human Brain (substantia nigra) lysates (calculated MW of 102kDa according to NP_005947.2). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb20061 (3.75µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb20061 (1µg/ml) staining of Jurkat (A), MCF7 (B) and (2ug/ml) MOLT4 (C) cel lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Western blot analysis of Human Substantia Nigra lysate using MTHFD1 antibody