MID2/TRIM1 Antibody

• Catalog Number: orb18730
• Category: Antibodies
• Description: Goat polyclonal antibody to MID2
• Target: MID2 / TRIM1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QESPYVSGMKTCH
• RRID: AB_10749468
• MW: 83.2, 79.9
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 5-10µg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Kidney shows strong staining in cytoplasm and brush border of some epithelial cells of proximal convoluted tubules. Recommended concentration, 5-10ug/ml.WB: Approx 80kDa band observed in mouse heart lysates (calculated MW of 81.3kDa according to NP_036348.1 and 77.9kDa according to NP_438112.1). Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti MID2 antibody, anti TRIM1 antibody, anti midl
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 11043

Western blot analysis of Mouse and Rat Heart, and Rat Kidney lysates using MID2 antibody

Immunohistochemical staining of Human Kidney using MID2 antibody