MEST Antibody

SKU: orb20256

Description

Goat polyclonal antibody to MEST

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:8000, WB: 0.2-0.5 μg/ml
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 37kDa band observed in Mouse and Rat Testis lysates (calculated MW of 37.6kDa according to Human NP_803490.1 and 38.8kDa according to Rat NP_001009617.1). Recommended concentration: 0.2-0.5 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: MEST
• Protein Sequence: QELLYRYKQNRSGR
• Molecular Weight: 38.8; 37.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti MEST antibody, anti mesoderm specific transcript homolog (mouse) antibody, anti DKFZp686L18234 antibody, anti MGC111102 antibody, anti MGC8703 antibody, anti PEG1 antibody, anti mesoderm specific transcript antibody, anti paternally expressed gene 1 antibody

orb20256 (0.2ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

MEST

NCBI Gene ID

4232