MCL1 Antibody

SKU: orb18789

Description

Goat polyclonal antibody to MCL1

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:2000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 35kDa band observed in lysates of Human Lung and cell line K562 (calculated MW of 37.3kDa according to NP_068779.1). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: MCL1
• Molecular Weight: 37.3; 21.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti Induced myeloid leukemia cell differentiation protein Mcl-1 antibody, anti mcl1/EAT antibody

orb18789 staining (1ug/ml) of Human Lung lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Quick Database Links

Gene Symbol

MCL1

NCBI Gene ID

4170