Goat anti-LXR alpha / LXR beta Antibody

• Catalog Number: orb18917
• Category: Antibodies
• Description: Goat polyclonal antibody to NR1H3; NR1H2
• Target: LXR alpha / LXR beta
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: CRLQDKKLPPLLSEI
• RRID: AB_10767252
• MW: 50.5; 50.1; 43.6; 51.1; 38.9
• Tested applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 10ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 55kDa band observed in human spleen lysates (calculated MW of 50.5kDa according to NP_005684 and 50.1kDa according to NP_009052). Recommended concentration: 1-3 μg/ml. Additional minor bands were also consistently observed at 38kDa and 75kDa. These bands were successfully blocked by incubation with the immunising peptide.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti LXRA antibody, anti LXR-a antibody, anti RLD-
• Note: For research use only
• Entrez: 10062, 7376

Western blot analysis of Human Liver (lane 1) and Kidney (Lane 2) lysate using NR1H3; NR1H2 antibody.

Immunofluorescence analysis of A549 cells, permeabilized with 0.15% Triton of NR1H3; NR1H2 antibody.

Flow Cytometry analysis of A549 cells of NR1H3; NR1H2 antibody.