LXR alpha/LXR beta Antibody

SKU: orb18917

Description

Goat polyclonal antibody to NR1H3; NR1H2

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 55kDa band observed in human spleen lysates (calculated MW of 50.5kDa according to NP_005684 and 50.1kDa according to NP_009052). Recommended concentration: 1-3 μg/ml. Additional minor bands were also consistently observed at 38kDa and 75kDa. These bands were successfully blocked by incubation with the immunising peptide.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: LXR alpha / LXR beta
• Protein Sequence: CRLQDKKLPPLLSEI
• Molecular Weight: 50.5; 50.1; 43.6; 51.1; 38.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti LXRA antibody, anti LXR-a antibody, anti RLD-1 antibody, anti NR1H3 antibody, anti nuclear receptor subfamily 1, group H, member 3 antibody, anti liver X receptor, alpha antibody, anti NR1H2 antibody, anti NER antibody, anti UNR antibody, anti LXR-b antibody, anti NER-I antibody, anti RIP15 antibody, anti nuclear receptor subfamily 1, group H, member 2 antibody, anti ubiquitously-expressed nuclear receptor / NR1H2 antibody, anti NER antibody, anti UNR antibody, anti LXR-b antibody, anti NER-I antibody, anti RIP15 antibody, anti nuclear receptor subfamily 1, group H, member 2 antibody, anti ubiquitously-expressed nuclear receptor antibody, anti OTTHUMP00000198038 antibody, anti liver X receptor alpha antibody, anti liver X receptor-alpha antibody, anti LXRB antibody, anti LX receptor beta antibody, anti iver X receptor beta antibody, anti liver X receptor-beta antibody, anti nuclear orphan receptor LXR-beta antibody, anti oxysterols receptor LXR-beta antibody

Western blot analysis of Human Liver (lane 1) and Kidney (Lane 2) lysate using NR1H3; NR1H2 antibody.

Immunofluorescence analysis of A549 cells, permeabilized with 0.15% Triton of NR1H3; NR1H2 antibody.

Flow Cytometry analysis of A549 cells of NR1H3; NR1H2 antibody.

Quick Database Links

Gene Symbol

LXR alpha / LXR beta

NCBI Gene ID

10062, 7376