LMP7 Antibody

SKU: orb20247

Description

Goat polyclonal antibody to PSMB8

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 0.01-0.03 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 22kDa band observed in Human Liver, Lung and Lymph Node lysates (calculated MW of 29.8kDa according to precursor NP_004150.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schwarz et al, J Immunol. 2000 Jul 15;165(2):768-78. PMID: 10878350. Recommended concentration: 0.01-0.03 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: LMP7
• Molecular Weight: 29.8; 30.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti PSMB8 antibody, anti proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7) antibody, anti D6S216 antibody, anti D6S216E antibody, anti LMP7 antibody, anti MGC1491 antibody, anti PSMB5i antibody, anti RING10 antibody, anti beta5i antibody, anti OTTHUMP00000029420 antibody, anti OTTHUMP00000029421 antibody, anti OTTHUMP00000062981 antibody, anti low molecular weight protein 7 antibody, anti macropain subunit C13 antibody, anti multicatalytic endopeptidase complex subunit C13 antibody, anti protease component C13 antibody, anti proteasome (prosome, macropain) subunit beta type 8 (large multifunctional protease 7) antibody, anti proteasome beta 8 subunit antibody, anti proteasome catalytic subunit 3i antibody, anti proteasome subunit Y2 antibody, anti proteasome subunit beta 5i antibody, anti proteasome-related gene 7 antibody

orb20247 (0.01ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

LMP7

NCBI Gene ID

5696