Goat anti-LEF1 Antibody

• Catalog Number: orb19285
• Category: Antibodies
• Description: Goat polyclonal antibody to LEF1
• Target: LEF1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QHEQRKEQEPKRPH
• RRID: AB_10748762
• MW: 44.2; 41.2; 42.7; 34.1
• Tested applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 48kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al , EMBO J. 2002 Jun 3;21(11):2703-14.; PMID: 12032083). Recommended concentration: 1-3 μg/ml.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti LEF1 antibody, anti lymphoid enhancer-binding
• Note: For research use only
• Entrez: 51176

Western blot analysis of MOLT4 lysate using LEF1 antibody