LEF1 Antibody

SKU: orb19285

Description

Goat polyclonal antibody to LEF1

Research Area

Epigenetics, Neuroscience, Signaling Pathways, Stem Cells, Wnt

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 48kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al , EMBO J. 2002 Jun 3;21(11):2703-14.; PMID: 12032083). Recommended concentration: 1-3 μg/ml.

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Key Properties

• Clonality: Polyclonal
• Target: LEF1
• Protein Sequence: QHEQRKEQEPKRPH
• Molecular Weight: 44.2; 41.2; 42.7; 34.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti LEF1 antibody, anti lymphoid enhancer-binding factor 1 antibody, anti HGNC:6551 antibody, anti DKFZp586H0919 antibody, anti TCF1ALPHA antibody, anti lymphoid enhancer binding factor-1 antibody

Quick Database Links

Gene Symbol

LEF1

NCBI Gene ID

51176