LASP1 (internal region) Antibody

SKU: orb19688

Description

Goat polyclonal antibody to LASP1

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.03-0.1 μg/ml, IHC-P: 2.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 36kDa band observed in Human Kidney lysates (calculated MW of 29.7kDa according to NP_006139.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreiber et al, Gene. 1998 Jan 30;207(2):171-5; PMID: 9511759). Recommended concentration: 0.03-0.1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: LASP1 (internal region)
• Protein Sequence: PERRDSQDGSSYR
• Molecular Weight: 29.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti LASP1 antibody, anti LIM and SH3 protein 1 antibody, anti Lasp-1 antibody, anti MLN50 antibody

orb19688 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Colon using LASP1 antibody

Immunohistochemical staining of Human Small Intestine using LASP1 antibody

Quick Database Links

Gene Symbol

LASP1 (internal region)

NCBI Gene ID

3927