KLF3/BKLF Antibody

SKU: orb18820

Description

Goat polyclonal antibody to KLF3

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 40kDa band observed in Human Heart lysates (predicted MW of 40kDa according to NP_057615). Recommended for use at 1-3 μg/ml. Please note that the 40kDa Band is sometimes accompanied by a 80-90kDa band of unknown identity.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: KLF3 / BKLF
• Protein Sequence: LMFDPVPVKQEAMD
• Molecular Weight: 38.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti KLF3 antibody, anti BKLF antibody, anti Kruppel-like factor 3 (basic) antibody, anti basic kruppel like factor antibody, anti MGC48279 antibody

Western blot analysis of Human Heart lysate using KLF3 antibody

Immunofluorescence analysis of A431 cells using KLF3 antibody

Immunofluorescence analysis of A549 cells using KLF3 antibody

Flow cytometric analysis of A431 cells using KLF3 antibody

Quick Database Links

Gene Symbol

KLF3 / BKLF

NCBI Gene ID

51274