JAM2/JAMB/CD322 Antibody

• Catalog Number: orb20275
• Category: Antibodies
• Description: Goat polyclonal antibody to JAM2
• Target: JAM2 / JAMB / CD322
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QRKGYFSKETSFQ
• RRID: AB_10939190
• MW: 33.2
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:4000, WB: 0.5-1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 35kDa band observed in Human Heart lysates (calculated MW of 33.2kDa according to NP_067042.1). Recommended concentration: 0.5-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti JAM2 antibody, anti junctional adhesion molec
• Research Area: Cardiovascular Disease
• Note: For research use only
• Entrez: 58494

orb20275 (0.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.