IRF2 Antibody

SKU: orb18405

Description

Goat polyclonal antibody to IRF2

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:2000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 48kDa band observed in lysates of cell line Jurkat (calculated MW of 39.3kDa according to NP_002190.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Li et al, Invest Ophthalmol Vis Sci. 1999 Apr;40(5):976-82.; PMID: 10102295. In transfected HEK293 transiently expressing IRF2 bands of approx. 40kDa and 45kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: IRF2
• Molecular Weight: 39.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti IRF2 antibody, anti interferon regulatory factor 2 antibody, anti DKFZp686F0244 antibody, anti IRF-2 antibody

Western blot analysis of HEK293 lysate using IRF2 antibody

Western blot analysis of Jurkat lysate using IRF2 antibody

Quick Database Links

Gene Symbol

IRF2

NCBI Gene ID

3660